From spirals to flagellar beating: How pivot-like defects control semiflexible filament dynamics in motility assays
Sandip Roy, Debasish Chaudhuri, Abhishek Chaudhuri
Published: 2025/9/1
Abstract
We investigate how localized pivot-like constraints regulate the dynamics of semiflexible filaments in two-dimensional motility assays. Motivated by rigor mutant motor proteins that remain bound but immobile, we model such constraints as pivot defects placed at arbitrary positions along the filament. Using large-scale simulations, we map out transitions between tightly coiled spirals and extended, flagella-like beating, controlled by pivot position, motor activity, and processivity. Spiral formation is characterized by bimodal turning number distributions and is stabilized by a balance between motor-driven activity and filament bending rigidity, with intermediate stiffness promoting robust spirals. We show that pivot defects act as an intermediate class of boundary condition, bridging free and clamped limits, and that their effects persist even when discretization is refined. While our model is minimal and does not include biochemical details, it provides a physically grounded framework for understanding how local pivot constraints reshape active polymer dynamics, yielding testable predictions for in-vitro molecular-motor assays